The Effect of ACTH4-10 synthetic on separated junction formation and CSF leukocyte number in LPS-induced meningitis

Mortality and morbidity in bacterial meningitis remain high in children and adults. Previous studies suggested that inflammatory response to bacterial products apparently continues even after the bactericidal effect of antibiotics, resulting in destruction of the host tissue. Previous studies showed that ACTH410 synthetic uses melanocortin receptors and it was considered that α-MSH shares the first 13 amino acids sequence with ACTH4-10, ACTH4-10 can be identified as α-MSH exogenous. αMSH has been known to have antiinflammatory activity. Research method used experimental studies on Wistar rats to compare expression of intracerebral inflammatory through the number of leukocytes in CSS and separated junctions formation between endothelial cells after intracisternal LPS challenge and after intranasal administration of ACTH4-10 .. The control group (n = 18) was given with LPS + Placebo and the experimental group (n = 18) was given with LPS + ACTH4-10. Inflammatory characteristics were measured and analyzed statistically. The results show the number of CSS leucocytes were significantly different (p <0.05) and there was a marked change in separated junction between the two groups (p <0.05). It can be concluded that in this study, ACTH4-10 synthetic administration significantly improved intracerebral inflammatory condition characterized by a decrease number of leukocytes and separated junction formation. Keywords– ACTH4-10 synthetic, LPS, Leukocyte, Meningitis, Separated Junction